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ผลงานตีพิมพ์ในวารสารวิชาการGenetic variation of avirulence genes (AVR-Pi9, AVR-Pik, AVR-Pita1) and genetic diversity of rice blast fungus, Pyricularia oryzae, in Thailandผู้แต่ง: Thanathip Sutthiphai, Katanyutita Damchuay, Ram Chandra Neupane, Apinya Longya,  Dr.Tanee Sreewongchai, Associate Professor , Dr.Pattavipha Songkumarn, Assistant Professor , Nonglak Parinthawong, KULCHANA DARWELL, Dr.Chatchawan Jantasuriyarat, Associate Professor , วารสาร: 
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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Effects of Plant Age on Symptom Development Produced by Pyricularia oryzae Toxin) ผู้เขียน:นายณรงค์ สิงห์บุระอุดม, รองศาสตราจารย์ , Ram Narayan Chaudhary, นายธรรมศักดิ์ สมมาตย์, ศาสตราจารย์ , ดร.เอ็จ สโรบล, ศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe main objective of this experiment was to determine suitable plant age for toxin inoculation on detached leaves and whole plant conditions. For detached leaves inoculation, it was found that 20 day old plants were the most sensitive to both crude extract toxin (CET) derived from culture filtrate of P. oryzae and tenuazonic acid. The degree of sensitivity was manifested by yellowing produced around the lesions of inoculated leaves. Forty day old plants were found to be the most appropriate age for CET inoculation as they produced typical blast symptom while tenuazonic acid could produce similar typical blast symptoms on leaves at all plant ages. The reactions to CET were varied to the different plant ages while the reactions to tenuazonic acid were not significantly different among them. The experimental result indicated that the lesion sizes produced on blast susceptible and resistant varieties upon CET and tenuazonic acid were not significantly different. In general, leaves exhibited higher sensitivity to CET than tenuazonic acid. It probably might be due to the concentration of the former higher than the latter. CET at concentration of 25% was the most appropriate for spray inoculation on 2-3 week-old seedlings. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Pathogenicity Determination of Crude Extract Toxin Produced by the Fungus Pyricularia oryzae Cav. Causal Agent of Rice Blast) ผู้เขียน:Ram Narayan Chaudhary, นายณรงค์ สิงห์บุระอุดม, รองศาสตราจารย์, นายธรรมศักดิ์ สมมาตย์, ศาสตราจารย์, ดร.เอ็จ สโรบล, ศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractSeries of experiments were carried out to determine the disease determinant factor of crude extract toxin produced by the rice blast fungus, Pyricularia oryzae, to determine an optimal concentration of crude extract toxin for screening against this toxin, and to obtain information on the phytotoxic effect of crude extract toxin on different varieties. The result indicated that crude extract toxin produced by P. oryzae was disease determinant factors. Leaf trated with this toxin produced spindle shaped to roundish lesion with gray centre and brown margin with or without halo and running lesion. The symptom was similar to those produced by the blast fungus infection. The capability of crude extract incited blast symptom increased when crude extract concentration increased. It was concluded that 25 percent concentration of the crude extract would be an appropriate concentration to inoculate rice plants. Genotypic variation was found among rice varieties against crude extract toxin inoculation. Varieties tested against crude extract toxin could be rated resistant, moderately resistant and susceptible to the toxin. The varieties produced small, roundish brown lesion with gray centre and without halo and running lesion were classified to be resistant whereas the varieties that produced larger round lesion with halo and running lesion were classified as susceptible. The result suggested that variety KTH-17 was considered to be susceptible whereas Usen was resistant. |
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หัวเรื่อง:ความสามารถในการก่อให้เกิดโรคของ culture filtrate ของเชื้อรา Pyricularia oryzae Cav. สาเหตุโรคใบไหม้ของข้าว ผู้เขียน:นายณรงค์ สิงห์บุระอุดม, รองศาสตราจารย์, Petcharat Chuntharathin, Vijai Rukvidhayasartra สื่อสิ่งพิมพ์:pdf AbstractCapability of culture filtrate peoduced by Pyricularia oryzae Cav. to incite blast symptom was conducted on rice variety KDML 105. Five isolates of the fungal pathogen were isolated from rice infected leaves. The result revealed that the symptoms produced by culture filtrate were quite similar to those produced by the fungal pathogen. The capabilities of culture filtrates for inciting disease symptoms were different among 5 isolates of the fungal pathogen. It might be concluded that culture filtrate containing toxic substance which was classified to pathotoxin could played an important role on virulent factor of blast pathogen. The response of rice leaves to culture filtrate was studied. The result indicated that as the increasing of culture filtrate concentrations, sizes and severity of lesions increased. The optimum incubation period for symptom appearance was 5 days after exposing culture filtrate to rice leaves. Rice varieties exhibited different responded to culture filtrate and their levels of response were determined by lesion sizes and lesion types. |
หัวเรื่อง:การตอบสนองของเนื้อเยื้อข้าวและแคลลัสต่อ culture filtrate เชื้อราMagnaporthe grisea (Pyricularia oryzae Cav.) ในขวดเพาะเลี้ยงเนื้อเยื้อ ผู้เขียน:นายณรงค์ สิงห์บุระอุดม, รองศาสตราจารย์, Petcharat Chuntharathin, Vijai Rukvidhayasartra, ดร.ประภา ศรีพิจิตต์, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe responses of rice tissue to culture filtrate of the fungus Magnaporthe grisea (Pyricularia oryzae Cav.), the causal agent of rice blast were investigated in vitro. Shoots and calluses of rice variety KDML105 were cultured on medium containing culture filtrate at concentrations of 0:9,1:9, 2:9, 3:9, 4:9 and 5:9 by volume, respectively. The result indicated that rice shoots exhibited significant difference response to different culture filtrate concentrations. Where the concentrations of culture filtrate increased, the height, number and survival of shoots decreased. At the concentration of 2:9, the height and number of shoots were reduced to 50 percent. The increasing of culturing period had an influence on the increasing of height and the number of shoots while the survival of shoots were reduced. Some of brown tissue could reproduce newly emerged shoots after culturing for a longer period.The responses of calluses to culture filtrate were investigated. The results revealed that where the concentrations of culture filtrate were increased, the calluses color became brown, while the diameter of calluses, amount of green spot formations and the number of regenerated shoots were reduced. At the concentration of 2:9, callus diameter was reduced to 50 percent, the callus color was changed to brown 50 percent, the percentage of green spot formations and the regenerated shoots were the highest at 3.6 and 12.5 percent, respectively. The result suggested that culturing period had a significant influence on callus development. On induction medium, while the culturing period was increased, the size of calli, degree of yellowing and green spot formations were increased whereas the color of calli was changed to brown, the number of survival was reduced and the number of regenerated shoots were increased when they were cultured on a regeneration medium. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Antagonism of Chaetomium globosum to the Rice Blast Pathogen, Pyricularia oryzae) ผู้เขียน:Kasem Soytong, T.H. Quimio สื่อสิ่งพิมพ์:pdf AbstractThe rice blast antagonist Chaetomium globosum isolated from upland rice field soils in the Philippines was antagonistic to Pyricularia oryzae. Coating rice seeds cv. IR442-2-58 with spore suspension (4 x 105 spore/ml) and culture extract from a specific strain of C. globosum resulted in preventing the early infection of rice blast pathogen. The non-treated seeds were as effective as captan. Benefits from coating rice seeds also increased emergence of shoots, greater fresh weight of plants, taller plants and lower disease incidence. The results suggest that screening for antagonism should not be limited to species only but should consider specific strains within species because antagonistic activity varied among strains species. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Inoculation Techniques of Crude Extract Toxin Produced by Pyricularia oryzae Cav) ผู้เขียน:Ram Narayan Chaudhary, นายณรงค์ สิงห์บุระอุดม, รองศาสตราจารย์, นายธรรมศักดิ์ สมมาตย์, ศาสตราจารย์, ดร.เอ็จ สโรบล, ศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe mechanical wounding of leaves is necessary for symptom development when inoculated with crude extract toxin (CET) as well as the most suitable method of inoculation for detached leaf and whole plant conditions. The experimental results revealed that the CET could produce disease symptoms even without leaf wounding before inoculation. For detached leaf condition, inoculation by micropipette was found to be efficient and effective. While foliar spray method was the most suitable for whole plant inoculation, symptoms produced by crude extract toxin varied to methods of inoculation. A 30 microlitres drop of CET by micropipette inoculation produced the similar typical blast symptom whereas spray inoculation produced small brown necrosis spots. CET spraying on detached leaves and whole plants gave the same result indicating that CET could be applied for screening toxin resistance on the whole plant condition. |
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ผลงานตีพิมพ์ในวารสารวิชาการGenetic diversity of an effector gene, AvrPi9, of rice blast pathogen in Thailand and characterization of its promoterผู้แต่ง:Damchuay, K., Hemsart, S., Longya, A., Leetanasaksakul, K., Kim, K.-T., Chung, H., Lim, Y.-J., Yoon, Y.-J., Lee, Y.-H., Toojinda, T., Dr.Chatchawan Jantasuriyarat, Associate Professor, วารสาร: 
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ผลงานตีพิมพ์ในวารสารวิชาการAssistance of phenotype-genotype selections for developing blast disease resistance of Thai jasmine rice, RD15ผู้แต่ง:Wangsawang, T., Waiyalert, A., Nonsiri, C., Dr.Prapa Sripichitt, Associate Professor, Dr.Pattavipha Songkumarn, Assistant Professor, Changsri, R., Cho, K.S., Dr.Tanee Sreewongchai, Associate Professor, วารสาร: |
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 Researcherดร. ฉัตรสุดา ศักดาเพชรศิริ, อาจารย์ที่ทำงาน:ภาควิชาโรคพืช คณะเกษตร กำแพงแสน สาขาที่สนใจ:Plant microbiome, Bioderagdation of xenobiotic substance, Bacterial Identification, Plant pathogenic bacteria, Bioformulation of biocontrol agents Resume |
 Researcherดร. ธิดา เดชฮวบ, รองศาสตราจารย์ที่ทำงาน:ภาควิชาโรคพืช คณะเกษตร สาขาที่สนใจ:สารเคมีป้องกันกำจัดโรคพืช (fungicides), ผลิตภัณฑ์ธรรมชาติจากจุลินทรีย์ควบคุมโรคพืช (Natural Products for Plant Protection), สารออกฤทธิ์ทางชีวภาพจากราเพื่อการควบคุมโรคพืช (biofungicide), อนุกรมวิธานและการจำแนกชนิดเชื้อรา (Taxonomy of Microfungi), สารทุติยภูมิจากเชื้อรา (Secondary Metabolites of fungi) Resume |